Wednesday, October 5, 2011

Patent Battle over Modified Meganucleases Heats Up

Meganucleases are endodeoxyribonucleases characterized by a large recognition site (double-stranded DNA sequences of 12 to 40 base pairs); as a result this site generally occurs only once in any given genome. For example, the 18-base pair sequence recognized by the I-SceI meganuclease would on average require a genome twenty times the size of the human genome to be found once by chance. Meganucleases are therefore considered to be the most specific naturally occurring restriction enzymes.

The high specificity of meganucleases gives them a high degree of precision and much lower cell toxicity than other naturally occurring restriction enzymes; they were identified in the 1990s as particularly promising tools for genome engineering.

However, the meganuclease-induced genetic recombinations that could be performed were limited by the repertoire of meganucleases available. Despite the existence of hundreds of meganucleases in nature, and the fact that each one is able to tolerate minor variations in its recognition site, the probability of finding a meganuclease able to cut a given gene at the desired location is extremely slim. Several research laboratories therefore soon began trying to engineer new meganucleases targeting the desired recognition sites.

Cellectis and Precision Biosciences are two competing biotechnology companies, both seeking to commercialize engineered meganucleases for use in genetic engineering.

On March 1, 2011, Cellectis sued Precision Biosciences in the District Court of Delaware for allegedly infringing US patent number 7,897,372, directed to "I-CreI Meganuclease Variants with Modified Specificity.” That case is presently proceeding with discovery, and trial is set for February 2013.

On September 20, 2011, the US patent office issued US patent number 8,021,867, which is directed towards "Rationally-Designed Meganucleases with Altered Sequence Specificity and DNA-Binding Affinity." The named assignee on the patent is Duke University, but according to Celectis the patent is actually owned by Precision.

On September 30, 2011, Cellectis filed another lawsuit against Precision Biosciences, again in the District Court of Delaware, and this time it is a declaratory judgment action seeking a declaration that the Duke/Precision patent is invalid and not infringed by Cellectis.

In the complaint, Cellectis does not allege that it has explicitly been threatened with a lawsuit by Precision. Instead, it argues that declaratory judgment is appropriate because: both patents-in-suit concern the same field of technology, i.e., "modified" or "altered" I-CreI meganucleases; the Cellectis patent is prior art to the precision patent, the parties in both cases are the same, and the discovery in both cases likely would substantially overlap.

12 comments:

how to get your ex back said...

Meganucleases specifically recognise large cleavage sites (>14 bp), and they are used in research labs to stimulate gene targeting events.

Oil and Gas Jobs Offshore said...

The road to success is always under construction”
Get it right….CIVIL ENGINEERS
A bridge is built for us to pass over; it is a work of utility, and which should endure. It should be in keeping with its object, solid, clean, simple, well executed without vain ornament.
Job oppurnity for nigerian peopole as well as international people, Offshore Engineering Jobs opportunity

collection accounts on credit report said...

I just love the way you work. Thanks for sharing this great and interesting stuff. Fabulous post! I really enjoyed that.

Penny stock newsletter said...

I have a web site where I research stocks under five dollars. I have many years of experience with these type of stocks. I would like to recommend a chinese stock that I think is attractive here the company Sutor Technology Group symbol [SUTR] Limited engages in the manufacture and sale of steel products primarily in the Peoples Republic of China. It offers hot-dip galvanized steel, prepainted galvanized steel, acid pickled steel, and cold-rolled steel products. The company sells its products to customers who operate primarily in the solar energy, appliances, automobile, construction, infrastructure, medical equipment, and water resource industries. It also exports its products to Europe, the Middle East, South America, the United States, southeast Asia, and Hong Kong. The company is based in Changshu City, China. the stock trades around 1.10 cents a share. I think the stock could get to 10.00 dollars a share over the next five years.

precision industries said...

hey nice blog, liked reading all the post and even i have a precision company which provides good quality as we believe in quality, we have a good name in Singapore market.

internet marketing belgium said...

amazing and a really a fun to read post

Project Finance Magazine said...

I really like your blog.. it it nice speech.

online gift to pakistan said...

nice blog thanks for sharing..

Flowers to Pakistan said...

I just like you writing style, Thanks for sharing this.

SEO Dubai said...

This is a really nice post.

birkin bag blue said...

Thanks for sharing the nice pictures. You have done a great work. Every homeowner can benefit from property management services.

Regional College Of Pharmacy said...

Your blog is very informative and gracefully
your guideline is very good.Thank you
Engineering college
Best engineering college in india
Private engineering college
pharmacy college
pharmacy college in india
top pharmacy college
Polytechnic college
Top polytechnic college
Top private polytechnic college
Top polytechnic colleges in India
Techanical colleges
Polytechnic college in india
Leading call center
Outbound call center india
Cheap Seo services india